| 质粒名称: | pAAV-hSyn-DIO-NES-SomaFRCaMPi-WPRE-bGH polyA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001219 |
| 产品用途: | Red calcium indicator for reporting neuronal activities |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn-DIO |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | FRCaMPi |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution
Zhou S., Zhu Q., Eom M., Fang S., Subach O.M., Ran C., Alvarado J.S., Sunkavalli P.S., Dong Y., Wang Y., Hu J., Zhang H., Wang Z., Sun X., Yang T., Mu Y., Yoon Y.G., Guo Z.V., Subach F.V., Piatkevich K.D.
PLOS Biol. 23, e3003048 (2025)
|
买家导航
pAAV-hSyn-DIO-NES-SomaFRCaMPi-WPRE-bGH polyA载体质粒基本信息
pAAV-hSyn-DIO-NES-SomaFRCaMPi-WPRE-bGH polyA质粒图谱载体图谱和pAAV-hSyn-DIO-NES-SomaFRCaMPi-WPRE-bGH polyA载体序列质粒序列多克隆位点信息
pAAV-hSyn-DIO-NES-SomaFRCaMPi-WPRE-bGH polyA质粒载体简介
pAAV-hSyn-DIO-NES-SomaFRCaMPi-WPRE-bGH polyA质粒序列载体序列
LOCUS pAAV-hSyn-DIO-NES-SomaFRCaMPi-WPRE-bGH polyA 6809 bp DNA circular SYN 26-MAY-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6809)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6809
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 12..141
/label=AAV2 ITR
promoter 165..612
/label=hSyn promoter
/note="human synapsin I promoter; confers neuron-specific
expression (Kugler et al., 2003)"
misc_feature 613..618
/label=SalI
protein_bind 625..658
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites."
protein_bind 709..742
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 751..755
/label=KpnI
CDS complement(757..2841)
/codon_start=1
/label=NES-SomaFRCaMPi
/translation="MLQLPPLERLTLSRSMVSKGEEDNMAIIKEFMRFKVHMEGSVNGH
EFEIEGEGEGRPYEAFQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFK
LSFPEGFRWERVMSFEDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGW
EASYEMTTRNLTDEQIAEFREAFSLLDRDQDGNITSNELGVVMRSLGLSPTAAELQDMI
NEVDADGNGTIDFTEFLTMMARKMKDTDDEEEVREAFKVFDKDGSGYITVEELTHVLTS
LGERLSREEVADVIREADSDGDGVINYEEFSRVISSKGGSGGSGSGSMRKPFRGAGNAV
RAFSTWKKLVPRVSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLPGA
YIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYKGGSGGSGGTGGSGGSGGTGG
SGGSGGTSGRTQISSSSFEFSSKVSRDTLYEAVREVLHGNQRKRRKFLETVELQISLKN
YDPQKDKRFSGTVRLKSTPRPKFSVCVLGDQQHCDEAKAVDIPHMDIEALKKLNKNKKL
VKKLAKKYDAFLASESLIKQIPRILGPGLNKAGKFPSLLTHNENMVAKVDEVKSTIKFQ
MKKVLCLAVAVGHVKMTDDELVYNIHLAVNFLVSLLKKNWQNVRALYIKSTMGKPQRLY
"
misc_feature 2847..2851
/label=NheI
protein_bind complement(2852..2885)
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites."
protein_bind complement(2936..2969)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 2970..2975
/label=EcoRI
misc_feature 2976..3564
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
misc_feature 3565..3570
/label=ClaI
polyA_signal 3573..4049
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
misc_feature 4050..4055
/label=PmlI
repeat_region 4072..4201
/label=AAV2 ITR
rep_origin 4287..4742
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5024..5128
/gene="bla"
/label=AmpR promoter
CDS 5129..5989
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6160..6748
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagaagtgca agtgggtttt
181 aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga cccactggac
241 aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac
301 aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc
361 ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc
421 ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca
481 gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc
541 ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc
601 ctgagagcgc aggtcgactc cggaataact tcgtatagga tactttatac gaagttatgc
661 agaatggtag ctggattgta gctgctatta gcaatatgaa acctcttaat aacttcgtat
721 agcatacatt atacgaagtt atggcgcggg ggtaccctaa tacagacgct ggggcttgcc
781 catggtgctc ttgatgtaca gagcccgcac gttttgccag tttttcttaa gcaaggacac
841 caagaaattg acagccagat gaatgttgta gactagctca tcatcggtca tcttcacgtg
901 gccaacagcg acggccaaac acagcacctt cttcatctgg aacttgattg tcgatttcac
961 ctcatccact ttggccacca tgttttcatt gtgtgtcagc agggagggga acttgccagc
1021 cttgtttagg cctgggccca ggatacgtgg gatctgctta atcagagact cagaggccaa
1081 aaaggcatcg tacttcttag ccagcttctt gaccaacttc ttgtttttgt taagtttctt
1141 gagcgcctcg atgtccatgt gggggatatc cacggccttg gcttcatcac agtgctgctg
1201 gtcccccaga acgcacaccg agaacttggg gcgtggggtg gacttgagcc tgacggtgcc
1261 cgagaaacgt ttgtccttct gagggtcgta gttcttcagg ctgatctgca gctccaccgt
1321 ctccagaaac ttgcggcgct tgcgctggtt cccgtgcagg acttcccgca ccgcctcgta
1381 cagggtgtcg cgtgagactt tgctgctgaa ctcgaagctt gagcttgaga tctgagtccg
1441 gcctgaggta cctccgctcc caccagatcc gccagtgcct ccgctccctc cagagccacc
1501 tgtccctccg ctaccgccag atccgccctt gtacagcgcg tccgtgccac cggtggggtg
1561 gcggcccacg gcgcgttcgc actgttccac gatggtgtag tcctcgttgt gggacaagat
1621 gtccaactcg atgtcgacga tgtaggcgcc gggcagctgc acgggcttct tggccttgta
1681 ggtggtcttg acctcggcgg cgtagtggcc gccgtccttc agcctcagcc ccttcctgat
1741 ctcgctcttc agggcgccgt cctcggggta catccactcg gaaacccgcg gcactagctt
1801 tttccaagtg ctgaaagcac gcacagcgtt gcccgcgcca cggaacggtt tcctcattga
1861 acctgacccg ctgcctcctg aaccgccctt ggaagaaatg acacgagaaa attcttcgta
1921 gttgattaca ccatcgccat cggagtcggc ttcacgtatc acatcggcta cttcttctcg
1981 agacaaacgt tcaccgagac ttgtaagaac atgagtcagc tcctcgactg taatgtatcc
2041 acttccatct ttatcgaaga ctttaaaggc ttcgcgaact tcctcttcgt cgtcggtatc
2101 cttcattttc cgggccatca ttgtcaaaaa ttcggtaaaa tcaattgtgc cattaccatc
2161 ggcatcgacc tcattaatca tatcttgtaa ttcggcggca gtaggcgata gacctaacga
2221 cctcataacc acacccaatt cattggacgt gatatttcca tcctgatcac gatcaagcag
2281 cgaaaaggcc tcacggaact ccgcaatctg ctcatctgta aggttacggg tagtcatctc
2341 gtaggaagcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg gggggaagtt
2401 ggtgccgagc agcttcacct tgtagatgaa tacgccgtcc tgcagggagg agtcctgatt
2461 aacgtgaata atgccgccgt cctcgaagct catcacgcgc tcccacctga agccctcggg
2521 gaaggacagc ttgaagtagt cggggatgtc ggctgggtgc ttaatgtagg ccttggagcc
2581 gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac
2641 cttcagccta gcggtctgga aggcctcgta ggggcggccc tcgccctcgc cctcgatctc
2701 gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat
2761 gatggccatg ttatcctcct cgcccttgct caccatagat ctcgaaagag taagacgttc
2821 aagaggagga agttgaagca tggtggctag cataacttcg tataaagtat cctatacgaa
2881 gttatttgcc ttaacccaga aattatcact gttattcttt agaatggtgc aaagaataac
2941 ttcgtataat gtatgctata cgaagttatg aattcaatca acctctggat tacaaaattt
3001 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
3061 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
3121 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
3181 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
3241 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
3301 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
3361 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
3421 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
3481 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
3541 tctccctttg ggccgcctcc ccgcatcgat acgggtggca tccctgtgac ccctccccag
3601 tgcctctcct ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat
3661 taagttgcat cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg
3721 tggtatggag caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg
3781 aaccaagctg gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc
3841 aagcgattct cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc
3901 tcagctaatt tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct
3961 ccaactccta atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg
4021 cgtgaaccac tgctcccttc cctgtccttc acgtgcggac cgagcggccg caggaacccc
4081 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
4141 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
4201 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
4261 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
4321 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
4381 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
4441 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
4501 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
4561 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
4621 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
4681 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
4741 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
4801 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
4861 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
4921 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
4981 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
5041 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
5101 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
5161 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
5221 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
5281 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
5341 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
5401 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
5461 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
5521 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
5581 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
5641 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
5701 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
5761 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
5821 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
5881 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
5941 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
6001 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
6061 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
6121 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
6181 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
6241 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
6301 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
6361 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
6421 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
6481 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
6541 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
6601 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
6661 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
6721 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
6781 ggccttttgc tggccttttg ctcacatgt
//
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